The potentiality of the functionalized nitrogen and thiol-doped graphene quantum dots (GQDs-N-S) to stabilize the antibodies in the designing of human chorionic gonadotropin immunosensor

摘要

In this study, for the first time, a simple immunosensor for ultrasensitive recognition of Human Chorionic Gonadotropin (hCG) in serum samples was fabricated by exploiting a simple approach. In this method, a low-cost and sensitive immunosensor was fabricated based on QDs-N-S/Au nanoparticles (NPs) modified Screen-Printed Carbon Electrode (SPCE). It seems that, QDs-N-S/Au NPs/ antibody as a biocomposite can be a good choice for the development of an impressive immunosensor. It must be said that in the modifying of immunosensor the length of the process have been reduced and the use of another substrates have been eliminated. pH and incubation time were optimized as two important parameters. Under the optimal conditions at modified SPCE, a linear communication in the range of 0.1 to 125 pg mL?1 and the detection limit of 12.5 fg mL?1 were obtained. The present procedure was utilized to the detection of hCG in real samples with the desirable results.