Protective effect of aqueous extract of Moringa oleifera Lam. stem bark on serum lipids, marker enzymes and heart antioxidants parameters in isoproterenol-induced cardiotoxicity in Wistar rats

摘要

Cardio protective potential of aqueous extract of Moringa oleifera Lam. (Family-Moringaceae) stem bark on serum lipids profiles, myocardial marker enzymes in serum and heart homogenate, antioxidant parameters in heart homogenate and histopathological findings in isoproterenol (ISO)-induced cardiotoxicity in rats was evaluated. Subcutaneous injection of ISO (85 mg/kg) to Wistar rats showed a significant ( P <0.05) increase in the levels of thiobarbituric acid reactive substances along with a significant decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione- S -transferase, glutathione reductase and reduced glutathione in the heart. It also significantly increased the level of cholesterol, triglycerides, VLDL and LDL with significant decrease in the level of HDL, increased the level of myocardial marker enzymes CK, LDH, ALT and AST in serum and concomitantly decreased in heart. Oral administration of aqueous extract of M. oleifera (250 and 500 mg/kg) to ISO-induced rats daily for a period of 21 days showed a significant improvement in lipid profile along with marker enzymes in serum and heart homogenate. It also decreased the levels of thiobarbituric acid reactive substances and improved antioxidant status by increasing the activities of antioxidant enzymes. Histopathological findings of the myocardial tissue showed the protective role of M. oleifera in ISO-induced cardiotoxicity in rats. The effect at 500 mg/kg dose was more pronounced.