Stable Somatic Gene Expression in Mouse Lungs Following Electroporation-mediated Tol2 Transposon Delivery

摘要

Gene delivery to the lung has rapidly progressed as an important method for studying various chronic lung diseases. Viral vectors, albeit highly efficient, are limited by the host immune response. Electroporation, a well - known non - vi ral method, can efficiently deliver genes to the lung, but is unable to induce stable gene expression. The Tol2 transposon is another non - viral method that can induce stable gene expression by reinserting its genes into the host genome. In this study, we c ombined electroporation and Tol2 transposons to obtain stable, high - level gene expression in the mouse lung. Tol2 transposon plasmids (pT2A - EGFP; Tol2, pCAGGS - TP; transposase) were optimized in vitro, and the electroporation procedure (pCAG - EGFP) was optim ized in mouse lungs. After optimization, a combination of electroporation plus the Tol2 transposon was used in a comparative analysis with electroporation plus pCAG - EGFP. GFP expression levels were quantified and visualized on days 4 and 7 post - electropora tion. We successfully reproduced the Tol2 transposon system in vitro and the electroporation procedure in vivo. We observed sustainable GFP expression using electroporation plus the Tol2 transposon on days 4 and 7, while electroporation plus pCAG - EGFP resu lted in decreased GFP expression on day 7. We were able to induce high - level, stable gene expression in mouse lungs using a combination of electroporation and the Tol2 transposon. This represents a safer method for lung gene delivery that can be used as an alternative to viral vectors.