Genetic and morphological variations among geographical populations of Rhopalosiphum padi (L.) (Hemiptera: Aphididae) in Egypt, using rapd and issr markers

摘要

 Morphological and genetic variation of sixteen geagraphical populations of Rhopalosiphum padi (L.) collected from different locality of Egypt, were studed by investgating eighteen morphometric or numeric morphological characters, applyingtwo molecular techniques; Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSRs) with whole genomic DNA of aphid. Moreover, phylogenetic relationships among those populations were also concerned on base of morphological or genetic variation. Most tested morphological characters were nearly constant among investigated populations except five characters.Population of El-Fayoum Governoratewas nearly different morphologically than others.The tested primers gave 47.27% polymorphism amongR. padigeographical populations.Arbitraryprimer C11 generated two unique bands with molecular weights 1400 and 1570 bp characterized Aswan and Menia populations, respectively. In addition,arbitrary primer (OPA-09) showed highest level of polymorphism at all(73.77%). Proximity matrix analysis, based on combined effect of RAPD- PCR and ISSRs, showed highest similarity value(85%)between geographical populations of Aswan and Sohag Governorates, while the lowest was with Qena and El-Sharqya populations. Moreover, the tested populations could be divided successfully into two main clusters, the first cluster include populations of Upper and Middle Egypt Governorates, while the second cluster includes populations of Lower Egypt Governorates