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首页> 外文期刊>Molecular biology of the cell >A Highlights from MBoC Selection: Megadalton-node assembly by binding of Skb1 to the membrane anchor Slf1
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A Highlights from MBoC Selection: Megadalton-node assembly by binding of Skb1 to the membrane anchor Slf1

机译:MBoC选择的亮点:通过Skb1与膜锚Slf1的结合形成Megadalton节点

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摘要

The plasma membrane contains both dynamic and static microdomains. Given the growing appreciation of cortical microdomains in cell biology, it is important to determine the organizational principles that underlie assembly of compartmentalized structures at the plasma membrane. The fission yeast plasma membrane is highly compartmentalized by distinct sets of cortical nodes, which control signaling for cell cycle progression and cytokinesis. The mitotic inhibitor Skb1 localizes to a set of cortical nodes that provide spatial control over signaling for entry into mitosis. However, it has been unclear whether these nodes contain other proteins and how they might be organized and tethered to the plasma membrane. Here we show that Skb1 forms nodes by interacting with the novel protein Slf1, which is a limiting factor for node formation in cells. Using quantitative fluorescence microscopy and in vitro assays, we demonstrate that Skb1-Slf1 nodes are megadalton structures that are anchored to the membrane by a lipid-binding region in the Slf1 C-terminus. We propose a mechanism for higher-order node formation by Skb1 and Slf1, with implications for macromolecular assemblies in diverse cell types.
机译:质膜包含动态和静态的微区。鉴于细胞生物学中皮质微区的日益增长,重要的是确定构成质膜分隔结构组装基础的组织原理。裂变酵母质膜被不同组的皮质节高度分隔,这些皮质节控制细胞周期进程和胞质分裂的信号传导。有丝分裂抑制剂Skb1定位于一组皮质结节,这些结节可对进入有丝分裂的信号提供空间控制。但是,目前尚不清楚这些节点是否包含其他蛋白质,以及它们如何组织并束缚在质膜上。在这里,我们显示Skb1通过与新型蛋白质Slf1相互作用形成结点,而Slf1是细胞中结点形成的限制因素。使用定量荧光显微镜和体外测定法,我们证明Skb1-Slf1节点是兆达尔顿结构,其通过Slf1 C端的脂质结合区锚定在膜上。我们提出了一种由Skb1和Slf1形成高阶节点的机制,对各种细胞类型中的高分子组装都具有影响。

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