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Specific expression of miR-17-5p and miR-127 in testicular and central nervous system diffuse large B-cell lymphoma

机译:miR-17-5p和miR-127在睾丸和中枢神经系统弥漫性大B细胞淋巴瘤中的特异性表达

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Recent studies have shown that certain non-coding short RNAs, called miRNAs, play an important role in diffuse large B-cell lymphomas. Patients with diffuse large B-cell lymphoma have great diversity in both clinical characteristics, site of presentation and outcome. The aim of our study is to validate the differential expression in germinal center and non-germinal center diffuse large B-cell lymphoma,s and to study to the extent to which the primary site of differentiation is associated with the miRNA expression profile. We studied 50 cases of de novo diffuse large B-cell lymphoma for the expression of 15 miRNAs (miR-15a, miR-15b, miR-16, miR-17-3p, miR-17-5p, miR-18a, miR-19a, miR-19b, miR-20a, miR-21, miR-92, miR-127, miR-155, miR-181a and miR-221). Apart from 19 nodal cases without extranodal dissemination (stages I and II), we selected two groups with unambiguous stages I and II extranodal presentation; 9 cases of primary central nervous system, 11 cases of primary testicular and 11 cases of other primary extranodal diffuse large B-cell lymphomas. All cases were analyzed with qRT-PCR. In situ hybridization for the most differentially expressed miRNAs was performed to show miRNA expression in tumor cells, but not in background cells. MiR-21 and miR-19b showed the highest expression levels. No significant differences were seen between germinal center and non-germinal center diffuse large B-cell lymphomas in either the total or the nodal group for any of the 15 miRNAs. Two miRNAs showed significant differences in expression levels for diffuse large B-cell lymphoma subgroups according to the site of presentation. MiR-17-5p showed a significant higher expression level in the central nervous system compared with testicular and nodal diffuse large B-cell lymphomas (PP<0.05). We conclude that the location of diffuse large B-cell lymphoma is an important factor in determining the differential expression of miRNAs.
机译:最近的研究表明,某些称为miRNA的非编码短RNA在弥漫性大B细胞淋巴瘤中起重要作用。弥漫性大B细胞淋巴瘤患者的临床特征,表现部位和预后都有很大差异。我们的研究目的是验证在生发中心和非生发中心弥漫性大B细胞淋巴瘤中的差异表达,并研究分化的主要部位与miRNA表达谱相关的程度。我们研究了50例新生扩散性大B细胞淋巴瘤中15种miRNA(miR-15a,miR-15b,miR-16,miR-17-3p,miR-17-5p,miR-18a,miR- 19a,miR-19b,miR-20a,miR-21,miR-92,miR-127,miR-155,miR-181a和miR-221)。除了19个没有淋巴结外扩散的淋巴结病例(I和II期),我们选择了I和II期淋巴结清晰的两组。原发性中枢神经系统9例,原发性睾丸11例,其他原发结外弥漫性大B细胞淋巴瘤11例。所有病例均采用qRT-PCR进行分析。进行了最差异表达的miRNA的原位杂交,以显示miRNA在肿瘤细胞中表达,但在背景细胞中不表达。 MiR-21和miR-19b显示最高的表达水平。在15个miRNA的全部或淋巴结组中,生发中心和非生发中心弥漫性大B细胞淋巴瘤之间均无显着差异。根据出现的位置,两个miRNA在弥散性大B细胞淋巴瘤亚组的表达水平上有显着差异。与睾丸和淋巴结弥漫性大B细胞淋巴瘤相比,MiR-17-5p在中枢神经系统中表达显着更高(PP <0.05)。我们得出结论,弥漫性大B细胞淋巴瘤的位置是决定miRNA差异表达的重要因素。

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