Expression of TPM1κ, a Novel Sarcomeric Isoform of theTPM1Gene, in Mouse Heart and Skeletal Muscle

摘要

We have investigated the expression of TPM1αand TPM1κin mouse striated muscles. TPM1αand TMP1κwere amplified from the cDNA of mouse heart by using conventional RT-PCR. We have cloned the PCR amplified DNA and determined the nucleotide sequences. Deduced amino acid sequences show that there are three amino acid changes in mouse exon 2a when compared with the human TPM1κ. However, the deduced amino acid sequences of human TPM1αand mouse TPM1αare identical. Conventional RT-PCR data as well as qRT-PCR data, calculating both absolute copy number and relative expression, revealed that the expression of TPM1κis significantly lower compared to TPM1αin both mouse heart and skeletal muscle. It was also found that the expression level of TPM1κtranscripts in mouse heart is higher than it is in skeletal muscle. To the best of our knowledge, this is the first report of the expression of TPM1κin mammalian skeletal muscle.