Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco Nicotiana Attenuata

摘要

AbstractNicotiana attenuata (N. attenuata) is an annual herb that induces nicotine, volatiles and phenolics in response to damage. In this study, we assembled the complete chloroplast (cp) genome of N. attenuata with the length of 155,886?bp, which was composed of a large single copy region (LSC) of 86,602?bp, a small copy (SSC) region of 18,518?bp and a pair of inverted repeats (IR) of 25,383?bp. This typical cp genome contains 112 unique genes, including 78 protein-coding genes, 30 tRNA genes and four rRNA genes. Phylogenetic analysis of 32 plant cp genomes based on 76 conserved protein-coding genes shows that N. attenuata is evolutionarily close to N. tabacum, then Capsicum annuum. The complete cp genome sequence of N. attenuata may provide a crucial foundation for the study of evolution, molecular biology and genetic engineering in Nicotiana species.