Methods of determing biokinetics of arsenate accumulation and release in Microcystis aeruginosa regulated by common environmental factors: Practical implications for enhanced bioremediation

摘要

Only little information is available on combined effects of abiotic environmental factors on algal arsenate (AsV) metabolic biokinetics. Herein, we demonstrated the methods of using the Taguchi statistical method to investigate four environmental factors includingAsV, nitrate (N), orthophosphate (P) and pH for their combined effects on algal growth and arsenic (As) uptake but also extracellular adsorption ofMicrocystis aeruginosa,as well asAsrelease from dead algal cells. Results showed that an increase of N facilitatedM. aeruginosagrowth and thus was the principal factor for the algal maximum specific growth rate (μmax). P was vital toAsVbioconcentration factor (BCF) andAspartition coefficients (LogKd) released from deal algal cells.AsVimpacted the extracellularAsadsorption onto the algal cells, which thereby increased with increasing initialAsVlevel. The initial pH had an imperative effect on theAsVuptake (ku) and release rate (Ke) from the dead cells. Collectively, the condition of low P, high N and alkaline pH level was favorable toAsaccumulation rate of living cells and restrictive toAsrelease rate from dead cells ofM. aeruginosa. The obtained information can pave a road for extensive understanding on efficient utilization ofAsbioremediation of algae in practical environment.?Principal factors were identified onAsVmetabolic biokinetics by Taguchi method.?High N and pH but low P fastenAsVuptake and reduceAsefflux from dead cells.?AsVonly as the main factor impactedAsextracellular adsorption on algal cells.