THE EFFECTOF SOME PESTICIDES ON ACETYLCHOLINESTERASE ACTIVITY FROM BOVINE BRAINE

摘要

Acetylcholinesterase (AChE) inhibitors are widely used for a variety of medical, agricultural and public health purposes. Consequently, exposure is highly possible during lifetime. Therefore, the aim of this study was to investigate in vitro the effects of pesticides such as cypermethrin, fenpropathrin, 1,1- D-methylpiperidinium chloride, N-(phosphonome- thyl)gylcine, P-naphthoxyacetic acid, lambda- cyhalothrin, deltamethrin, and 2,4-dichoroplenoxy acetic acid on AChE activity obtained from bovine braine. Pesticides showed different effects on AChE activity. The AChE enzyme was inhibited by cyper- methrin, fenpropathrin, 1,1-D-methylpiperidinium chloride, N-(phosphonomethyl)gylcine, and P-naph- thoxyacetic acid pesticides and activated by lambda- cyhalothrin and deltamethrin pesticides. 2,4-dichor- oplenoxy acetic acid pesticide did not alter the activity of AChE enzyme. AChE activity was inhibited in a concentration-dependent manner, and calculated IC50 values for cypermethrin (IC50=24.45 mM), fenpropathrin (IC50=16.95 mM), 1,1-D-methylpiper- idinium chloride (IC50=69.09 mM), N-(phospho- nomethyl)gylcine (IC50=5 85 mM), and P-naphthox- yacetic acid (IC50=16.34 mM).