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首页> 外文期刊>Fresenius environmental bulletin >EFFICIENT, CHEAP AND RELIABLE METHOD FOR THE EXTRACTION OF gDNA FROM THE HARD AND COMPLEX TISSUES OF COCONUT
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EFFICIENT, CHEAP AND RELIABLE METHOD FOR THE EXTRACTION OF gDNA FROM THE HARD AND COMPLEX TISSUES OF COCONUT

机译:从椰子的复杂而复杂的组织中提取gDNA的高效,廉价和可靠的方法

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摘要

Here we explained a convenient, rapid and cheap method of gDNA extraction from the hard and complex tissues of the coconut. The method has also been verified against betel nut and available vegetables (mint, spring onion, radish, ginger, coriander) at the Coconut Research Institute, Wen- chang-China. Extraction of gDNA was carried out through 8 simple steps. The method doesn't require intensive cleaning steps as designated to the commercially available kits. The method efficiently isolated the highly intact gDNA with great purity. This cheap and reliable method has broad future prospects in the field of plant and animal genetics, breeding and pathology.
机译:在这里,我们解释了从椰子的硬组织和复杂组织中提取gDNA的便捷,快速和廉价的方法。该方法也已在中国文昌椰子研究所针对槟榔和可用蔬菜(薄荷,葱,萝卜,姜,香菜)进行了验证。 gDNA的提取通过8个简单步骤进行。该方法不需要市售套件指定的密集清洁步骤。该方法以高纯度有效分离了高度完整的gDNA。这种便宜可靠的方法在动植物遗传学,育种和病理学领域具有广阔的前景。

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