SIMULTANEOUS AND RAPID ANALYSIS OF INTRACELLULAR POLYMER DURING DENITRIFYING PHOSPHORUS REMOVAL USING NEAR INFRARED SPECTROSCOPY AND CHEMOMETRICS

摘要

To realize simultaneous and rapid analysis of the intracellular polymer during denitrifying phosphorus removal, near infrared spectroscopy and BP neutral network algorithm are used to established the analytical models (BP neutral network calibration models) of the poly-β-hydroxybutyrate (PHB), polyphosphate (Poly-P) and glycogen (Gly). After the stability of denitrifying phosphorus removal process, 100 samples were used to establish BP neutral network calibration models, and 40 samples were used to test the performance of the established models. Multiple scatter correction was used as a spectral preprocessing method. The preprocessing results showed that multiple scatter correction can effectively eliminate the baseline shift and migration of near infrared spectra that result from scattering, and the signal-to-noise ratio of near infrared spectral data is improved. preprocessed spectral data were used to establish the BP neutral network calibration models of PHB, Poly-P and Gly. The BP neutral network calibration models of PHB, Poly-P and Gly showed that the correlation coefficients (rc) were respectively 0.9840, 0.9473 and 0.9283, with the root mean square errors of cross validation (RMSECV) being 0.0051, 0.0057 and 0.0063 respectively. In addition, the test results of the BP neutral network calibration models of PHB, Poly-P and Gly indicated that the correlation coefficient (rp) were respectively 0.9789, 0.9291, 0.9182, with the root mean square errors of prediction (RMSEP) being 0.0057, 0.0073 and 0.0080. It showed that BP neutral network calibration models can better analyze PHB, Poly-P and Gly during denitrifying phosphorus removal. The research suggests that near infrared spectroscopy and BP neutral network algorithm may provide a simultaneous and rapid analysis of intracellular polymer.