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NEXT GENERATION SEQUENCING TECHNOLOGIES AND USAGE IN FORENSIC GENETICS

机译:下一代测序技术及其在法医学中的应用

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DNA sequence analysis applications, which started, all the way back in the 90s and have stepped up with the Human Genome Project, has reached a completely different level with the techniques and methods developed nowadays. Almost all fields of application have taken advantage of these developments where single base level and genome distributed DNA sequence differences can be detected in maternal twins [1]. Forensic Genetics did not keep distance from these developments that started with clinical purpose and with the New Generation Sequencing techniques developed in recent years, it is stated that a new era in this field has begun. Particularly the convenience provided by the analysis of mix samples containing DNA of multiple persons and due to presenting more than one genetic markers in a single analysis, these technologies have brought great dynamism to the field. Once the foremost disadvantages of New Generation Sequencing methods situated as the cost that comes with high technology and analysis errors of high amounts of information produced are overcome, a paradigm shift is inevitable in where ^length polymorphism^ dominates the field of forensic genetics [2].
机译:DNA序列分析应用程序从90年代就开始使用,并随着人类基因组计划的发展而发展起来,与当今开发的技术和方法已经达到了完全不同的水平。几乎所有的应用领域都利用了这些发展优势,其中在母体双胞胎中可以检测到单碱基水平和基因组分布的DNA序列差异[1]。法医遗传学与这些从临床目的开始的发展以及最近几年开发的新一代测序技术之间的距离并不遥远,这表明该领域的新时代已经开始。特别是通过分析包含多个人的DNA的混合样本所提供的便利,并且由于在一次分析中提供了多个遗传标记,这些技术为该领域带来了极大的活力。一旦克服了新一代测序方法的最主要缺点,即高科技带来的成本和大量信息产生的分析错误,就不可避免地发生了范式转变,其中“长度多态性”主导了法医遗传学领域[2]。 。

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