Dezvoltarea si validarea unei metode HPLC pentru determinarea metronidazolului, oxitetraciclinei si furazolidonei din produse farmaceutice

摘要

An isocratic reversed phase high performance liquid chromatographic method with DAD detection wasdeveloped for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phaseconsisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70:18:12). The UV detectionwas carried out at 264 nm, and the flow rate was 1.100 mL/min. The separation was carried out on aNucleosil C18 column, 5 μm 250 mmx4.6 mm, which was maintained at 25 0C. This method was validatedby system suitability parameters, linearity, limits of detection and quantification, precision and accuracy.