ISOLATION AND UTILIZATION OF A TRICLOPYR DEGRADING BACTERIUM, BACILLUS CEREUS TR-13

摘要

A strain TR-13 capable of highly degrading triclopyr was isolated from soil. The strain TR-13 was identified as Bacillus cereus based on 16S rRNA and analysis of morphology, physiological and biochemical characters. The optimal pH value and temperature were 7.0-7.5 and 30-35^. The 7d degradation rate of 100 mg/L triclopyr by strain TR-13 could reach about 98.5% in inorganic salt medium. The strain TR-13 could hydrolyze triclopyr to 3,5,6-tri-chloro-2-pyridinol(TCP) ,but could not degrade TCP as the sole carbon source for its growth. The combined applications of the strain TR-13 and the strain CM-Z6 was considered an effective means for degrading triclopyr, because the strain CM-Z6 could degrade TCP. The degradation rate of triclopyr (100mg/L) by the strain TR-13 and the strain CM-Z6 could reach about 99% in 6d. In addition, the strain TR-13 could tolerate 800 mg/L of triclopyr and showed a high tolerance to triclopyr.