OPTIMIZATION OF MEDIUM CONTENTS BY EXPERIMENTAL DESIGN FOR PRODUCTION OF THE ANTIMICROBIAL SUBSTANCE FROM AIRBORNE Streptomyces sp.

摘要

The aim of this study was to optimize antibiotic production from airborne actinomycete, Streptomyces sp. isolated from atmosphere of Eskisehir, using experimental design as statistical method. The Plackett-Burman screening method was evaluated as a means to assess the significance chosen eleven factors, including starch, glucose, glycerol, casein, soybean peptone, meat peptone, K2HPO4, KH2PO4, MgSO4, NaCl, and CaCO3. The Plackett-Burman design showed that three factors (starch, casein and soybean peptone) were the most convenient contents as carbon and protein sources for biosynthesis of antimicrobial metabolites. To find appropriate concentration of these factors were varied at three levels in 27 experiments using full factorial design. The results of analysis obtained by using SPSS statistical software, noted that the optimum combination of the three factors for maximum efficiency of the antibiotic determined as follows concentration of carbon source (starch) 10g/l, concentration of nitrogen sources (soybean peptone and casein) 5g/l.