Effect of septic shock on lipolytic activity in rats' blood and adipose tissue

摘要

The metabolic study of septic shock indicates an intensification of anaerobic glycolysis, gluconeogenesis, proteolysis and limitation of lipid utilisation as a source of energy. The object of this study is the influence of a septic shock on lipolytic activity in the rats` blood and adipose tissue. The septic shock was developed by intraperitoneal injection of bacterial suspension (107 E. coli). The blood was taken in the hyperdynamic (I phase) and hypodynamic (II phase) period of a septic shock and the levels of glucose, insulin, free fatty acids (FFA), triglycerides (TG), total cholesterol (TC) and its fractions (HDL, LDL), lipolytic activity of serum (LAS) and lipid mobilising activity (LMA) were determined. Results: the glucose concentration in blood was increased in phase I of the septic shock to 8.70±1.30 mmol/l, in phase II it was decreased to 2.93±0.42 mmol/l. The insulin level of blood serum was increased to 16.15±3.60 μU/ml in phase I of the septic shock, in phase II, the insulin level was decreased to the level which was observed in the control group (8.15±1.71 μU/ml). The concentration of serum TC progressively decreased during the septic shock, as well as the HDL-cholesterol concentration. The concentration of the LDL-cholesterol fraction was increased to 0.26±0.01 mmol/l in phase I, and in phase II it decreased to 0.14±0.03 mmol/l. We demonstrated the increase of serum TG to 1.93±0.65 mmol/l with its reduction of FFA to 0.37±0.06 mmol/l in phase I of the septic shock. In phase II, the TG concentration decreased to 1.34±0.12 mmol/l, but it was still higher than in the control group (0.92±0.10 mmol/l) and the FFA concentration was increased to 1.22±0.26 mmol/l. In phase I of the septic shock, the LAS was increased to 1.88±0.52 μm/ml/h, in phase II it was decreased to 0.02±0.01 μm/ml/h. The LMA progressively increased during the septic shock and in phase II it reached 0.21±0.07 μm/ml/h.