Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B 1 Detection

摘要

An impedimetric immunosensor for fumonisin B 1 (FB 1 ) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB 1 immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB 1 immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L ?1 , and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng ?1 and 3.8 pg·L ?1 , respectively. The limit of detection of the immunosensor for certified corn sample ( i.e. , certified reference material) is 0.014 ppm FB 1 , which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA).