Reclamation of Marine Chitinous Materials for the Production of ???±-Glucosidase Inhibitors via Microbial Conversion

摘要

Six kinds of chitinous materials have been used as sole carbonitrogen (C/N) sources for producing ???±-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) as the C/N source. The half maximal inhibitory concentration (IC 50 ) and maximum aGI activity of fermented deCSP (38 ???μg/mL, 98%), deSSP (108 ???μg/mL, 89%), squid pen powder (SPP) (422 ???μg/mL, 98%), and shrimp head powder (SHP) (455 ???μg/mL, 92%) were compared with those of fermented nutrient broth (FNB) (81 ???μg/mL, 93%) and acarbose (1095 ???μg/mL, 74%), a commercial antidiabetic drug. The result of the protein/chitin ratio on aGI production showed that the optimal ratio was 0.2/1. Fermented deCSP showed lower IC 50 and higher maximum inhibitory activity than those of acarbose against rat intestinal ???±-glucosidase.