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首页> 外文期刊>Marine Drugs >An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes
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An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

机译:源自邦纳木霉的乙醇提取物可清除紫外线B(UVB)辐射诱导的活性氧种类,并减轻UVB诱导的人类角质形成细胞的细胞损伤。

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The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280–320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.
机译:本研究调查了来自红藻海棠的乙醇提取物对人HaCaT角质形成细胞中紫外线B(UVB)诱导的细胞损伤的保护作用。 Bonnemaisonia hamifera乙醇提取物(BHE)清除了黄嘌呤/黄嘌呤氧化酶系统生成的超氧阴离子和Fenton反应生成的羟基自由基(FeSO 4 + H 2 O 2 ),两者均通过电子自旋共振光谱法检测。此外,BHE表现出对过氧化氢或UVB辐射诱导的1,1-二苯基-2-甲基苯并肼基自由基和细胞内活性氧(ROS)的清除活性。 BHE减少了UVB诱导的凋亡,如凋亡小体形成减少和DNA片段化所表明。 BHE还减弱了UVB介导的氧化应激导致的DNA损伤以及8-异前列腺素和蛋白质羰基的升高水平。此外,BHE吸收了UVB范围(280-320 nm)内的电磁辐射。这些结果表明,BHE通过清除ROS和吸收UVB光子来保护人类HaCaT角质形成细胞免受UVB诱导的氧化损伤,从而减少对细胞成分的伤害。

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