Innovative Approach to Sustainable Marine Invertebrate Chemistry and a Scale-Up Technology for Open Marine Ecosystems

摘要

Isolation of marine compounds from living invertebrates represents a major challenge for sustainable and environmentally friendly exploitation of marine bio-resources. To develop innovative technology to trap invertebrate compounds in the open sea, the proof of concept of a system combining external continuous circulation of water with XAD-amberlite solid-phase extraction was validated in an aquarium. In this work, we reported the elicitation of guanidine alkaloid production of Crambe crambe in the presence of Anemonia sulcata , both collected from the Mediterranean Sea. Besides the previously reported crambescidin 359 ( 1 ), and crambescidin acid ( 2 ), three new compounds were isolated; one carboxylated analog of 1 named crambescidin 401 ( 3 ), and two analogs of crambescin B, crambescin B 281 ( 4 ) and crambescin B 253 ( 5 ). Based on these results, a technology named Somartex ? for “Self Operating MARine Trapping Extractor” was patented and built to transfer the concept from closed aquarium systems to open marine ecosystems.