Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase ( DOG R1 ) gene as a selectable marker for oil palm ( Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

摘要

DOG~(R)1 , which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG~(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l~(-1)2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOG~(R)1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOG~(R)1 gene and 2-DOG for regenerating transgenic oil palm.