Comparative Analysis of Immune Activation Markers of CD8 + T Cells in Lymph Nodes of Different Origins in SIV-Infected Chinese Rhesus Macaques

摘要

Altered T-cell homeostasis, such as expansion of CD8~(+)T cells to the secondary lymphatic compartments, has been suggested as a mechanism of HIV/simian immunodeficiency virus (SIV)-pathogenesis. However, the role of immune activation of CD8~(+)T cells in the CD4/CD8 turnover and viral replication in these tissues is not completely understood. In this study, we compared the expression of immune activation markers (CD69 and HLA-DR) on CD8~(+)T cells in the peripheral blood and lymph nodes (LNs) of SIV-infected/uninfected Chinese rhesus macaques. SIV-infected macaques had significantly higher percentages of CD8~(+)CD69~(+)and CD8~(+)HLA-DR~(+)T cells in all these anatomical compartments than uninfected macaques. LNs that located close to the gastrointestinal (GI) tract (colon, mesenteric, and iliac LNs) of SIV-infected macaques had profoundly lower numbers of CD4~(+)T cells, but no significant difference in expression of activation marker (CD8~(+)CD69~(+)and CD8~(+)HLA-DR~(+)) as compared with the peripheral lymphatic tissues (axillary and inguinal LNs). The CD4/CD8 ratios were negatively correlated with the activation of CD8~(+)T cells in the overall LNs, with further associations with CD8~(+)HLA-DR~(+)in GI LNs while CD8~(+)CD69~(+)in peripheral LNs. These observations demonstrate that the increase of CD8~(+)T cell activation is a contributing factor for the decline of CD4/CD8 ratios in GI system.