Diagnosis of Acute Infection with Bovine Viral Diarrhea Virus in Dairy Cattle by Using Indirect ELISA and Immunohistochemistry Technique on Skin Biopsy Samples

摘要

Diagnosis of Bovine Viral Diarrhea Virus infection (BVDV) is an important step in control anderadication programs in cattle. Acute infection can result in deliver a persistent infected (PI) calf, secondaryinfection to BVD-linked immunosuppression or reproductive losses. The present work was carried out on a totalnumber of 80 dairy Frisian cattle. Serum samples were examined for BVDV antibodies by using indirect EnzymeLinked Immunosorbent assay (ELISA). Ear notch tissue samples were taken for immunohistochemistry (IHC)by using strept avidin-biotin peroxidase complex method. A total number of 12 ear notch skin biopsy with hightitre of BVDV IgG gave positive IHC results. Immunopositive reaction was observed as golden brown intracytoplasmicgranules which were distinctive in cells of stratum germinativum and stratum spinosum ofepidermis as well as hair follicles. Placenta of early aborted cases showed multiple areas of extensivecoaggulative necrosis, inflammatory reaction and numerous clumps of bacterial colonies of mixed types. It couldbe concluded that indirect ELISA assay and IHC techniques on ear notch skin biopsy are efficient and reliablemethods for detection of acute form of BVDV infection in cattle.