首页> 外文期刊>G3: Genes, Genomes, Genetics >Sex Determination in Ceratopteris richardii Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte
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Sex Determination in Ceratopteris richardii Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte

机译:拟南芥中的性别决定是伴随着转录组的变化,驱动年轻配子体的表观遗传重编程。

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The fern Ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to de novo assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This C. richardii gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants.
机译:蕨蕨(Ceratopteris richardii)是研究同型植物性别决定和配子分化的重要模型。在这里,我们使用RNA序列从头组装一个转录组,并鉴定年轻配子体中差异表达的基因,因为它们的性别取决于是否存在称为雄激素的雄激素信息素。在鉴定出的1,163个共有差异表达基因中,绝大多数(1,030个)在用anteridiogen处理的配子体中上调。这些DEG的GO术语富集分析表明,与配子体基因组的表观遗传重编程有关的大量基因被信息素上调。额外的激素反应和发育基因也被信息素上调。该RICHARDii配子体转录组和基因表达数据集将被证明对侧重于植物的性别决定和分化的研究有用。

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