Effect of You-Gui-Wan on endometrial angiogenesis in controlled ovarian hyperstimulation mice model

摘要

Purpose: To explore the mechanism of You-Gui-Wan (YGW) in improving endometrial receptivity in mouse model of controlled ovarian hyperstimulation (COH). Methods: Sixty female mice with two consecutive oestrous cycles were randomly divided into COH model group (n = 50) and normal control group (n = 10). The COH model was created by using GnRH-a + PMSG + HCG and further randomly divided into 5 subgroups of simple model, YGW high-dose, YGW medium-dose, YGW low-dose and aspirin control, 10 in each subgroup. YGW or aspirin or saline was administrated to the mice by gavage once a day for 11 days. Afterwards, blood and uterine samples were collected for examination of serum levels of estradiol (E2) and progesterone (P4), endometrial thickness and number of blood vessels as well as expressions of angiogenic factors of VEGF, Ang-1, Ang-2 and Tie-2 at transcription and translation levels by using H& E staining, ELISA, real-time qRT-PCR, and immunohistochemistry, respectively. Results: COH per se increased the serum concentrations of E2 and P4???but decreased the number of endometrial blood vessels and the protein expressions of the angiogenic factors when compared to the normal control group (P 0.05 or 0.01). YGW increased the mRNA expressions of all angiogenic factors and restored the effect of COH on number of endometrial blood vessels (P 0.01), and the protein expressions of the angiogenic factors when compared to the COH simple model group (P 0.05 or 0.01). In comparison, aspirin could only restore the number of endometrial blood vessels and the protein expressions of Ang-2 and Tie-2 (P 0.05 or 0.01). Conclusions: YGW can restore the side effect of COH on number of endometrial blood vessels and the expressions of angiogenic factors, thus improve endometrial receptivity in COH mice.