Analytical sequence to study G-CSF effect on the transcriptome of isolated spinal motoneurons from {SOD1} {G93A} mice, an animal model for amyotrophic lateral sclerosis

摘要

Abstract Granulocyte-colony stimulating factor (G-CSF) has been recently identified as a neurotrophic factor able to preserve motor functions, rescue motor units and extent survival in an animal model of amyotrophic lateral sclerosis, the {SOD1} {G93A} mice. To gain insight into the mode of action of G-CSF, we have recently performed gene expression profiling on isolated lumbar motoneurons from {SOD1G93A} mice, and shown that G-CSF re-adjusted gene expression in motoneurons of symptomatic {SOD1G93A} mice and modulates genes related to neuromuscular function (Henriques et al., 2015). Here, we provide quality controls for the microarray experiment (GO accession number GSE60856) and describe the experimental strategy.