Genome-wide {DNA} binding pattern of two-component system response regulator RhpR in Pseudomonas syringae

摘要

Abstract Although Pseudomonas syringae uses the two-component system RhpRS to modulate the expression of type {III} secretion system (T3SS) genes and pathogenicity, the molecular mechanisms and the regulon of RhpRS have yet to be fully demonstrated. We have performed a genome-wide analysis of RhpR binding to {DNA} prepared from P. syringae pv. phaseolicola in order to identify candidate direct targets of RhpR-mediated transcriptional regulation, as described in our recent article [1]. The data are available from {NCBI} Gene Expression Omnibus (GEO) with the accession number GSE58533. Here we describe the detailed methods and data analyses of our RhpR ChIP-seq dataset.