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Demarcation of informative chromosomes in tropical sweet corn inbred lines using microsatellite DNA markers

机译:利用微卫星DNA标记划分热带甜玉米自交系信息染色体。

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A study of genetic variation among 10 pairs of chromosomes extracted from 13 tropical sweet corn inbred lines, using 99 microsatellite markers, revealed a wide range of genetic diversity. Allelic richness and the number of effective alleles per chromosome ranged from 2.78 to 4.33 and 1.96 to 3.47, respectively, with respective mean values of 3.62 and 2.73. According to the Shannon's information index (I) and Nei's gene diversity coefficient (Nei), Chromosome 10 was the most informative chromosome (I = 1.311 and Nei = 0.703), while Chromosome 2 possessed the least (I = 0.762 and Nei = 0.456). Based on linkage disequilibrium (LD) measurements for loci less than 50 cM apart on the same chromosome, all loci on Chromosomes 1, 6 and 7 were in equilibrium. Even so, there was a high proportion of genetic variation in Chromosomes 4, 5, 8, 9 and 10, thereby revealing their appropriateness for use in the genetic diversity investigations among tropical sweet corn lines. Chromosome 4, with the highest number of loci in linkage disequilibrium, was considered the best for marker-phenotype association and QTL mapping, followed by Chromosomes 5, 8, 9 and 10.
机译:利用99个微卫星标记,对从13个热带甜玉米自交系中提取的10对染色体之间的遗传变异进行了研究,揭示了广泛的遗传多样性。等位基因丰富度和每条染色体有效等位基因的数量分别在2.78至4.33和1.96至3.47之间,平均值分别为3.62和2.73。根据Shannon信息指数(I)和Nei的基因多样性系数(Nei),第10号染色体是信息最丰富的染色体(I = 1.311和Nei = 0.703),而第2号染色体的信息染色体最少(I = 0.762和Nei = 0.456)。 。根据同一染色体上相距少于50 cM的基因座的连锁不平衡(LD)测量,染色体1、6和7上的所有基因座均处于平衡状态。即便如此,在第4、5、8、9和10号染色体中仍有很高比例的遗传变异,从而揭示了它们适合用于热带甜玉米品系的遗传多样性研究。连锁不平衡位点数量最多的4号染色体被认为最适合标记-表型关联和QTL作图,其次是5号,8号,9号和10号染色体。

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