Genodiversity of dominant Rhizobium leguminosarum bv. Trifolii isolated from 11 types of soil in Serbia

摘要

Rhizobium leguminosarum bv. trifolii is microsymbiont Trifolium pratense and Trifolium repens, which are very important legumes in Serbia. The natural nodulating population of those bacteria was collected and estimated biodiversity distribution by monitoring dominant genotypes of these bacteria. The population of Rhizobium leguminosarum bv. trifolii were collected from 50 marked locations of 11 types of soil in Serbia. 437 natural isolates, rescued from nodules of Trifolium repens or Trifolium pratense, were analyzed by phenotypic approach. We obtained 156 different isolates on the basis of differences in their IAR - intrinsic antibiotic resistance (five antibiotics) and HMT- heavy methal tolerance (five heavy metals). We investigated 56 dominant isolates with more than three differences in IAR-HMT patterns by REP-PCR and RAPD fingerprinting (AP10 and SPH 1 primers). The results showed genodiversity of dominant Rhizobium leguminosarum bv. trifolii field isolates and offered the possibility to assess their changes on marked locations during time and under different environmental conditions and geographical distribution.