Sleep deprivation affects all aspects of health. Adverse health effects by sleep deviation are still underestimatedand undervalued in clinical practice and, to a much greater extent in monitoring human health. We hypothesizedthat sleep deprivation-induced mild organ injuries; oxidative stress and inflammation might play a crucial role ininducing multi-organ injury. Male C57BL/6J mice (n = 6-7) were sleep-deprived for 0-72 h using a modifiedmultiple platform boxes method. Blood and tissue were collected. Liver, heart, kidney, lung, and pancreatic injurieswere evaluated using biochemical and histological analyses. Glutamic oxaloacetic transaminase (GOT), glutamicpyruvic transaminase (GPT), total billirubin (TBIL), creatine phosphokinase (CPK), creatine phosphokinase-myocardial band (CKMB), lactic dehydrogenase (LDH), creatinine (CRE), and blood urea nitrogen (BUN)were assayed in blood. Malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor (TNF)-?, interleukin(IL)-1?, and IL-6 levels were measured. Histology revealed mild-to-moderate liver and lung injury in sleepdeprivedmice. Sleep-deprived mice had significantly higher GOT, GPT, TBIL, CPK, CKMB, LDH, BUN, and?-amylase (AMYL) levels, which indicated liver, heart, kidney, and pancreatic injuries. Serum IL-1? at 24 h andIL-6 at 72 h were significantly higher in sleep-deprived than in control mice. Hepatic TNF-? and IL-1? weresignificantly higher, but IL-6 significantly lower in mice that had been sleep-deprived for 72 h. Sleep deprivation-mediated inflammation may be associated with mild to moderate multi-organ damage in mice. The implicationof this study indicates sleep deprivation in humans may induce multi-organ injury that negatively affectscardiovascular and gastrointestinal health.
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