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首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Development of a Biocrystallisation Assay for Examining Effects of Homeopathic Preparations Using Cress Seedlings
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Development of a Biocrystallisation Assay for Examining Effects of Homeopathic Preparations Using Cress Seedlings

机译:生物结晶测定方法的开发,用于检验使用水芹苗的顺势疗法制剂的效果

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A major challenge in basic research into homeopathic potentisation is to develop bioassays that yield consistent results. We evaluated the potential of a seedling-biocrystallisation method. Cress seeds (Lepidium sativumL.) germinated and grew for 4 daysin vitroinStannum metallicum30x or water 30x in blinded and randomized assignment. 15 experiments were performed at two laboratories. CuCl2-biocrystallisation of seedlings extracted in the homeopathic preparations was performed on circular glass plates. Resulting biocrystallograms were analysed by computerized textural image analysis. All texture analysis variables analysed yielded significant results for the homeopathic treatment; thus the texture of the biocrystallograms of homeopathically treated cress exhibited specific characteristics. Two texture analysis variables yielded differences between the internal replicates, most probably due to a processing order effect. There were only minor differences between the results of the two laboratories. The biocrystallisation method seems to be a promising complementary outcome measure for plant bioassays investigating effects of homeopathic preparations.
机译:顺势疗法潜能基础研究的主要挑战是开发能够产生一致结果的生物测定法。我们评估了幼苗生物结晶法的潜力。水芹种子(Lepidium sativumL。)发芽并在盲孔和随机分配下在金属锡30x或水30x中体外生长4天。在两个实验室进行了15个实验。在顺势疗法制剂中提取的幼苗的CuCl2生物结晶在圆形玻璃板上进行。通过计算机化的纹理图像分析来分析所得的生物晶体图。分析的所有质地分析变量均对顺势疗法产生了显着结果;因此,经过顺势疗法处理的水芹的生物晶体图结构表现出特定的特征。两个纹理分析变量在内部重复之间产生差异,这很可能是由于加工顺序的影响。两个实验室的结果之间只有很小的差异。对于研究顺势疗法制剂效果的植物生物测定法,生物结晶法似乎是一种有前途的补充结果测量方法。

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