Qualitative and quantitative study on bacterial flora of farm raised common carp, Cyprinus carpio in India

摘要

Analysis of pond water and sediment as well as skin and intestine of common carp, cultured under polycultre system, was done quantitatively and qualitatively. During the study of 60 days (winter and summer seasons), total viable counts of bacteria were in the range of 4.43±0.50x103 to 5.5 ±0.09x103 cfu g-1 and 7.43±0.03x103 to 9.66±0.09x103 cfu g-1, respectively in water of A, B and C ponds. In the sediment, bacterial biomass during winter was in the range of 3.23 ±0.06x104 to 4.46± 0.15x104 cfu g-1 and during summer it was found to be in the range of 8.3 0.26x104 cfu g-1 to 9.43± 0.24 x104 cfu g-1. During winter and summer phase, bacterial biomass in skin and intestine was 3.16 0.09 x103 cfu g-1 to 3.56 0.12x103 cfu g-1 and 6.03 0.20 x105-106 cfu g-1 to 7.76 0.20 x105-106 cfu g-1 respectively for all 3 replicates. In total, 10 bacterial genera and 13 dominant species were identified. The bacteria of pond water and sediment reflected the bacterial composition in skin, gill and intestine of the fish. In the pond water, Corynebacterium spp., Aeromonas hydrophila, Pseudomonas spp., Achromobacter sp. and Flavobacter spp. were predominant whereas in pond sediment, Aeromonas hydrophila, Pseudomonas spp., Corynebacterium sp., Flavobacter spp. and Bacillus sp. were predominant and Corynebacterium spp., Aeromonas hydrophila, Flavobacter spp. and Pseudomonas spp. were predominant in skin, gills and intestine of common carp. During the experimental period, Pseudomonas aeruginosa, Aeromonas hydrophila, Flavobacter devorans and Corynebacterium sp. were predominantly present in all the samples in all the phases.