转基因鲤鱼与对照鲤肠道微生物群落差异研究

摘要

以转"全鱼"生长激素基因鲤(Cyprinus carpio L.)和野生对照鲤为对象,采用454高通量测序技术对其肠道微生物16S rRNA基因进行测序并分析了3个不同发育阶段微生物群落结构的变化, 进而探讨了转基因鲤与对照鲤肠道微生物群落的差异.基于转基因鲤和对照鲤不同发育时期(6日龄、2月龄、5月龄)肠道微生物群落组成的DCA排序分析显示, 2月龄转基因鲤与对照鲤肠道微生物组成不同.Alpha多样性及均匀度都显示转基因鲤肠道微生物多样性高于对照鲤.从门水平的比较分析发现, 转基因鲤肠道中存在较多的厚壁菌门(Firmicutes)细菌, 而对照鲤中拟杆菌门(Bacteroidetes)细菌较多, 其中2月龄转基因鲤肠道内Bacteroidetes/Firmicutes比值低于对照鲤.研究结果表明, 在所分析的3个发育时期, 转基因鲤的肠道微生物组成与对照鲤相比发生了改变, 且在2月龄时存在差异.该研究为进一步揭示转基因鱼肠道微生物与宿主的相互影响和作用机制提供了很好的参考.%To investigate the intestinal bacterial communities differences between the transgenic common carp and con-trols, carp intestinal bacterial community at different developmental stages (6 day, 2 month, 5 month) were used for 454 high throughput sequencing. Detrended correspondence analysis (DCA) showed that the intestinal bacterial communi-ties of transgenic common carp and controls at 2 month age were significantly different. Alpha diversity and evenness results indicated that gut microbial diversity of transgenic common carp was higher than that of the control. We found that transgenic carp gut had more bacteria belonging to Firmicutes, and the controls had more bacteria belonging Bac-teroidetes. The Bacteroidetes/Firmicutes ratio of transgenic common carp was significantly lower than that of controls in 2 month age. All data showed that transgenic common carp had different intestinal bacterial composition compared with wild controls at three developmental stages, especially at 2 month stage. Our findings provide insight into the in-teraction and mechanism between microbes and the host.