Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot FungusFomes fomentariusin Solid-State Fermentation

摘要

Statistical approaches were employed for the optimization of different cultural parameters for the production of laccase by the white rot fungusFomes fomentariusMUCL 35117 in wheat bran-based solid medium. first, screening of production parameters was performed using an asymmetrical design 2533//16, and the variables with statistically significant effects on laccase production were identified. Second, inoculum size, CaCl2concentration, CuSO4concentration, and incubation time were selected for further optimization studies using a Hoke design. The application of the response surface methodology allows us to determine a set of optimal conditions (CaCl2, 5.5 mg/gs, CuSO4, 2.5 mg/gs, inoculum size, 3 fungal discs (6 mm Ø), and 13 days of static cultivation). Experiments carried out under these conditions led to a laccase production yield of 150 U/g dry substrate.