Restriction Fragment Length Polymorphism and Gene Mapping of Two Genes Associated with Milk Composition in Egyptian River Buffalo

摘要

Gene maps and genetic polymorphism s of genes related to the economic quantitative traits as tools for developing more efficient breeding strategies have as targeted animal improvement by the genomic approach. This study aimed to identify the genetic polymorphism and gene mapping of two genes, BTN and ODC, are associated with milk composition in river buffalo. By means of PCR, the amplified fragment of BTN obtained from all tested buffalo DNA was at 501 bp. After digestion of the PCR products with HaeIII, we can distinguish between A and B alleles. The allele A is the most common allele in the Egyptian river buffalo, where the estimated frequencies were 0.89 and 0.11 for alleles A and B, respectively. The PCR products of ODC with a 796 bp fragment were digested with MspI. Fifty four of 70 animals (77.14%) have MspI(+/+) genotype and 13 animals (18.57%) have MspI(+/-) whereas three animals (4.29%) displayed the homologous MspI(-/-) genotype. The assignment of BTN and ODC to river buffalo chromosomes was done by calculating the correlation coefficient (Y) between these two genes and markers representing the river buffalo chromosomes. The results showed that BTN is assigned to the bi-armed buffalo chromosome 2, whereas ODC is assigned to the acrocentric river buffalo chromosome 12.