An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carriers

摘要

BackgroundYarrowia lipolyticais a nonconventional, dimorphic yeast with multiple biotechnological applications. Considering the size ofY. lipolyticacells and a plethora of its morphological forms (spherical cells or hyphae and pseudohyphae), it is highly difficult to select a suitable carrier for this useful microorganism. Bacterial cellulose (BC) is currently considered one of the most promising immobilization carriers. In the current study, the usefulness of oil- and emulsion-modified BCs as a carrier forY. lipolyticaimmobilization was investigated. Static and agitated cultures were conducted in media supplemented with oil or emulsion to improve carrier porosity.ResultsIt was found that the application of oil- and emulsion-modified BCs correlated with significantly higher efficiency ofY. lipolyticaimmobilization and hence higher yield than the yield achieved with an unmodified carrier. Increased efficiency of immobilization correlated with BC porosity-related parameters, which, in turn, depended on the size of oil droplets introduced into the culture medium. Moreover, changes in porosity-related parameters caused by the addition of oil or emulsion to the medium were observed when the cultures were conducted only under static conditions and not under agitated conditions.ConclusionThe application of oil- and emulsion-modified BCs as carriers significantly increased the efficiency ofY. lipolyticaimmobilization as compared to unmodified BC. The addition of oil or emulsion to the culture medium can be a simple but effective method to modify the porosity of BC-based carriers.How to cite:?ywicka A, Wenelska K, Junka A, et al. An efficient method ofYarrowia lipolyticaimmobilization using oil- and emulsion-modified bacterial cellulose carrier. Electron J Biotechnol 2019;41.https://doi.org/10.1016/j.ejbt.2019.06.004.