Abstract The aim of the present study was to obtain up-to-date information on the distribution of helminths in buffalo farms of central Italy. The survey was carried out on a sample of 127 farms (epidemiological units), selected using a grid approach within a Geographical Information System, followed by proportional allocation. In each farm the number of buffaloes tested (adults, heifer/steers and calves) ranged from 9 to 21 based on the number of animals on the farm. The total number of faecal samples collected from the 127 farms was 1883. Copromicroscopic examinations were performed using the FLOTACtechnique. The following helminths were detected in the examined farms: gastrointestinal strongyles (33.1%), Strongyloides spp. (3.1%), Fasciola hepatica (7.1%), Dicrocoelium dendriticum (2.4%), Paramphistomidae (7.1%), and Moniezia spp. (2.4%).
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