Efficiency of FAME analysis with SP-2380 capillary column in lamb meat

摘要

Abstract The healthiness and flavour of meat are largely related to its fat content and fatty acids (FAs) composition. In ruminant tissues an important role is played by the isomers of octadecadienoic acids (the most abundant of which are cis 9 trans 11 and trans 11 cis 12) as well as the cis and trans isomers of C18:1w9 and C18:2w6. It is very useful separate them together with the other FAs methyl esters (FAMEs) by a single run, as performed by capillary column highly polar. In this work, GC/MS analyses were performed with a Varian system (Saturn 2100T) and the separation was achieved on a SP-2380 (Supelco, Bellafonte, PA, USA) with stabilised poly (90% biscyanopropyl/10% cyanopropylphenyl siloxane) as a phase, with L=60m, I.D. 0.25mm, d_f=0.20 _m. According to the best separation and fastest analysis times, the temperature program was the following: initial 50°C, hold 5 min, ramp at 10°C/min, final 240°C, hold 2 min (with hold 8 min at 230°C). The method allowed selective and simultaneous determination of a wide range of different FAMEs in lamb meat, thereby eliminating many of the hassles associated with food analyses. The calibration of GC/MS analysis was performed using the solution mix Fim-Fame-7 Mixture (Matreya, Inc., Pleasant Gap, PA) as external standard, contains 37 components and with total concentration of 30mg/ml in methylene chloride. Six standard mix solutions (1.5, 3.0, 4.5, 5.0, 15 and 30 mg/ml) were prepared and injected in quadruple to test the linearity of the response, which resulted significant for all the FAMEs examined, with correlation coefficients R2 ≥ 0.9998. The slopes vary in a narrow range from 0.9665 to 1.0662; C14:0 and C14:1w9 had lower slopes, and thus are detected with a lower sensitivity, than the other esters.