Protective Role of Pomegranate Peel Extract on Testis in Adult Male Rabbits Treated with Carbon Tetrachloride

摘要

The aim of the present study is to prepare ethanol extract of Pomegranate peel and the effects ofthis extract on testicular weight to body weight ratio, Serum cholesterol, testosterone concentrationand histopathological changes of testes in rabbits treated with carbon tetrachloride (CCl4). Twentyfour adult male rabbits were used. They were divided randomly into four equal groups. Animalswere treated for 56 days as following: Rabbits of the 1st group were received 1 ml distal water orallyonce a day and olive oil 0.5 ml /kg B.W. I.P twice a week as control group. The second group weretreated I.P with 500mg / kg B.W. of CCl4 mixing with equal volume of olive oil (0.5 ml/kg B.W.)twice a week (group T1). The third group was received pomegranate peel extract orally(100 mg/kg B.W) once a day and olive oil 0.5 ml /kg B.W. I.P twice a week (group T2). The fourthgroup were received pomegranate peel extract (100 mg/kg B.W) once a day oral I.P with 500 mg /kg B.W. of CCl4 mixing with equal volume of olive oil (o.5 ml/kg B.W.) twice a week (group T3).Blood samples were collected at (0, 14, 28, 42 and 56) days for measuring testosteroneconcentration, Serum cholesterol after treatments. Animals weighed and scarified and testis wereremoved and weighed, Samples of testis were taken for histopathological study. The results of thepresent study showed that treatment with pomegranate peel extract causes a significant (P>0.05)increase in testicular weight to body weight ratio. Also a significant (P>0.05) decreased of serumcholesterol and a significantly (P>0.05) elevation of testosterone concentration were observed.Histopathological examination of the testis was revealed that the extract of Pomegranate peelprotect the testis against lesions caused by CCl4. In conclusion, Pomegranate peel extract couldprotect the tissue of testicles from CCl4 perhaps, by its anti-oxidative effect of pomegranate peelextract, hence eliminating the deleterious effects or toxic effect of CCl4