Accumulation and Elimination of Enrofloxacin and Ciprofloxacin in Tissues of ShrimpLitopenaeus vannameiunder Laboratory and Farm Conditions

摘要

This study aimed to quantify the accumulation and elimination of Enrofloxacin (ENRO) and Ciprofloxacin (CIPRO) in cultivatedLitopenaeus vannameiunder controlled laboratory and farm conditions. Laboratory- and farm-raised shrimp were given feed supplemented with 200 mg/kg ENRO for 14 days, followed by a 16-day diet without antibiotics. The levels of ENRO and CIPRO were analyzed by High Performance Liquid Chromatography (HPLC). In the laboratory, ENRO concentrations in the muscle and hepatopancreas reached a maximum (Cmax) of0.54±0.26 μg/g and3.52±1.9 μg/g, respectively;Cmaxvalues for CIPRO in the laboratory were0.18±0.13 μg/g (muscle) and1.05±0.20 μg/g (hepatopancreas). In farmed shrimp,Cmaxvalues for ENRO were0.36±0.17 μg/g muscle and1.60±0.82 μg/g in the hepatopancreas; CIPROCmaxvalues were0.03±0.02 μg/g (muscle) and0.36±0.08 μg/g (hepatopancreas). Two to fourteen days were necessary to eliminate both antibiotics from muscular tissue and four to more fourteen days for complete elimination of the antibiotics from the hepatopancreas. These results should be considered in terms of minimum concentrations necessary to inhibitVibriobacteria to determine whether the current use of this antibiotic is effective in controlling disease.