Potato Virus Y (PVY) is one of the most important viruses affecting cultivated potatoes in Egypt. Different potato plants were collected from an experimental station in Giza Governorate, Egypt and were tested using RT-PCR. PVY was amplified using primers represented portion of the Coat Protein (CP) gene and 3' Untranslated Regions (UTR). Phylogenetic tree showed two main strain groups: Group I regroups PVYN and PVYNTN stains while Group II includes PVYO, PVYW and PVYN:O strains. The Egyptian PVY isolate was clearly classified within group I and was more closely related to PVYNTN strains. Ten nucleotide substitutions resulted in 3 conserved amino acid substitutions (V1→I, G7→E, M or V and S8→G) and were able to differentiate between both groups. The partial coat protein region was more diverse than that of the 3'UTR (92.6-100% and 97.7-100% identity, respectively). The 3'UTR of the Egyptian isolate showed RNA secondary structures different from those of the 5 PVY strains.
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机译：马铃薯病毒Y（PVY）是影响埃及栽培马铃薯的最重要病毒之一。从埃及吉萨省的一个实验站收集了不同的马铃薯植株，并使用RT-PCR进行了测试。使用代表外壳蛋白（CP）基因部分和3'非翻译区（UTR）的引物扩增PVY。系统发育树显示了两个主要的菌株组：第一组重组PVY N 和PVY NTN 染色，而第二组包括PVY O ，PVY W 和PVY N：O 菌株。埃及PVY分离株明确地归入第一类，并且与PVY NTN 菌株更密切相关。十个核苷酸取代导致3个保守氨基酸取代（V 1 →I，G 7 →E，M或V和S 8 →G ），并能够区分两组。外壳蛋白的部分区域比3'UTR的区域更多样化（分别为92.6-100％和97.7-100％同一性）。埃及分离株的3'UTR显示出不同于5个PVY菌株的RNA二级结构。
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