Utilization of prune juice or puree as a laxative for constipation pregnant rats induced iron intake during pregnancy and the impact on newborns

摘要

Iron supplementation during pregnancy causes constipation in pregnant women and newborn babies; especially on high doses. Prune juice and prune puree were tested to avoid the constipation in pregnant women and newborn babies. Phenolic compounds, vitamin C and sugar alcohols of prune juice and prune puree were determined. Forty two pregnant female rats fed a basal diet for 6 weeks. At the 10th day of pregnancy they were divided randomly into 7 groups. Group 1, was the negative control (N. control). Rats from group 2 to group 4 fed on basal diet and received 1mg Fe~(2+)/kg b.w. Group 3 and group 4 administered 0.5 ml prune juice and prune puree twice daily, respectively. Moreover, rats from group 5 to group 7 fed on basal diet and received 2.5 mg Fe~(2+)/kg b.w. Group 6 and group 7 administered 0.5 ml prune juice and prune puree twice daily, respectively. Group 2 and 5 were considered positive control 1 and 2, respectively. The mothers rats were received Fe~(2+) (ferrous fumarate dosage form) daily until parturition; however, they were administrated prune juice and prune puree until weaning at the end of experiment (42 days). The mothers of rats and puppies were killed at the end of breast feeding. The results of HPLC-UV detector indicated that quantification of phenolic compounds, vitamin C, sorbitol and xylitol in prune puree were higher than prune juice. Concerning the results of biological experiment, it could be noticed that Fe~(2+) (ferrous fumarate form) significantly decreased the pellet number, weight, and % moisture of feces in pregnancy, lactating, in male and female infant rats. Moreover, gastrointestinal movements were significantly reduced in rats administrated with 1 or 2.5mg Fe~(2+)/Kg b.w represented in the G2 and G5 compared to the negative control (G1). Moreover, prune juice and prune puree utilization improved the tested parameters for pregnancy, lactating and infant rats whether they were male or female. The results clearly showed that prune juice or puree can prevent or treat constipation via normalizing the bowel movement resulting from Fe~(2+) administration in pregnant rats and their puppies (newborn and infant).