IDENTIFICATION, QUANTIFICATION AND VALIDATION OF β-SITOSTEROL FROM HOLOPTELEA INTEGRIFOLIA ( Roxb.) PLANCH USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

摘要

Objective: The Present study was designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromato graphic (HPTLC) method for the determination of beta‐sitosterol in methanolic leaf extract of holoptelea integrifolia (Roxb.) Planch. Methods: As per ICH guidelines we have applied different concentrations of beta‐sitosterol as standard on HPTLC plates for the quantification of beta sitosterol from the plant extract. Concentration of standard beta‐sitosterol was 0.1μg/μl Results: The retention factor of beta‐sitosterol was 0.38. Linearity was obtained in the range of 100 ng‐500 ng for beta sitosterol. The developed and validated HPTLC method was employed for beta‐sitosterol in methanolic leaf extract of holoptelea integrifolia (Roxb.) Planch for Standardization of the content of the marker. Satisfactory recoveries of 85.0 ‐86.0 % were obtained for beta‐sitosterol. Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of beta‐sitosterol in methanolic leaf extract of holoptelea integrifolia (Roxb.) Planch