PHYTOCHEMICAL ANALYSIS, HEPATOPROTECTIVE AND HPTLC ANALYSIS OF FRACTIONS OF ERYTHROXYLUM MONOGYNUM METHANOLIC LEAF EXTRACT ON PARACETAMOL INDUCED HEPATIC DAMAGE

摘要

Objective: To investigate the hepatoprotective activity of fractions of E. monogynum methanolic leaf extract against paracetamol induced hepatic damage and perform HPTLC analysis of active fraction. Methods: Fractions derived from methanolic extract of leaves of E. monogynum (Pet. ether, chloroform and hydroalcoholic) were screened for hepatoprotective activity. Doses of 100mg/kg, 200mg/kg of different fractions administered for seven days and on 8 th day toxicity by paracetamol was induced. Levels of biochemical markers along with histopathological changes were monitored to evaluate the extent of hepatoprotection after 24 hrs of toxicity induction. Results: A significant decrease in the biochemical parameters was evident by the hydroalcoholic fraction as compared to the toxic group which is also confirmed by histopathological changes observed. HPTLC analysis of hydroalcoholic fraction confirmed the presence of rutin, a flavonoidal glycosides besides other phytochemicals. Conclusion : The results of the present study indicates the significant hepatoprotective activity by the hydroalcoholic fraction of E. monogynum against paracetamol induced toxicity. Keywords : Erythroxylum monogynum , Hepatoprotection, Paracetamol, Silymarin, HPTLC.