COMPARATIVE ANALYSIS OF TOTAL FLAVONOIDS AND QUERCETIN CONTENT IN VIVO AND IN VITRO AND ENHANCEMENT OFQUERCETIN VIA PRECURSOR FEEDING INPLUCHEA LANCEOLATA OLIVER & HIERN

摘要

Objective: The present investigation deals with the comparative analysis of total flavonoids and quercetin content in vivo (leaf, stem, and root) and in vitro(unorganised static callus) plant parts as well as the enhancement of quercetinvia precursor feeding in callus cultures of Pluchealanceolata. Methods: Unorganised static callus culture was established from young leaf explants onMurashige and Skoog's (MS) basal medium supplemented with NAA (1.0 mg/l) in combination with BAP (0.5 mg/l). Isolation and identification of bioactive secondary metabolite quercetin was carried out by the colour reaction, TLC behaviour, IR spectrum, and HPLC techniques.Enhancement of quercetin in callus cultures was achieved by the addition of various concentration of precursor/s (phenylalanine and cinnamic acid) in culture media. Result: Total flavonoids was found to be maximum (25.7 mg/g dry wt) in leaves. Maximum quercetincontent (0.23 mg/g dry wt) was obtained in 6 weeks old callus tissues. Exogenous incorporation of cinnamic acid (5 mg/100ml) increased the yield of total quercetin about 7 to 8 fold in comparison to control (control = 0.23mg/g dry wt; fed=1.86 mg/g dry wt), whereas incorporation of phenylalanine (50 mg/100ml)increased the yield of total quercetinonly 1 to 2 fold in comparison to control(control=0.23mg/g dry wt; fed=0.38mg/g dry wt). Therefore, cinnamic acid is a more effective precursor than phenylalanine. Conclusion: Hence,the present study proves that theaccumulation of quercetin in callus cultures can be exploited for its large-scale production in a possible array of health promoting benefits as anti-oxidant, anti-inflammatory and anti-cancerous agents in future