PHYTOCHEMIMCAL ANALYSIS OF WRIGHTIA TINCTORIA BARK EXTRACT IN WATER USING GC-MS

摘要

Objective: In the present study, phytochemical constituents of crude water extract of Wrightia tinctoria (WT) bark is done using GC-MS technique. Wrightia tinctoria is an important medicinal herb being used in tribal areas of Chhattisgarh since long but chemical constituents of its bark responsible for the activities are still not studied in depth. Methods : Dried bark powder was successively extracted with petroleum ether, ethyl acetate and methanol using soxhlet apparatus and lastly material was dissolved in distilled water for 10 hrs for extraction. Water extract was selected for the further analysis using Agilent 7890A GC with 5975MS. Results : As per the GC-MS analysis, twelve different compounds namely benzene 1, 2, 4, 5-tetramethyl (2.85%), benzene 1, 2, 3, 5-tetramethyl (1.16%), 1-decanol, 2, 2-dimethyl (4.38%), phenol 2, 4-bis (1, 1-dimethyl ethyl) (7.78%), heptadecane (3.60%), 3-hexadecanol (3.30%), i-propyl tetradecanol (3.64%), benzo (h) quinoline (3.66%), n-hexadecanoic acid (6.54%), octadecanoic acid methyl ester (0.81%), phytane (1.95%) and pentadecane (2.25%) were characterized. Analysis and identification of presence of the compound in these extracts were done using the database of NIST library. Conclusion: Study confirms the presence of biologically active phyto-constituent in water extract of Wrightia tinctoria bark those may be the key chemical of natural origin in new drugs designing against major disease those are being treated in tribal areas using this plant bark. Further confirmation of in vitro bioactivity using cell line culture is required which is planned as the future prospect of current study. Keywords: Wrightia tinctoria, Phytochemical, GC-MS analysis, Apocyanaceae.