OPTIMIZATION OF CULTURAL PARAMETERS FOR ANTIFUNGAL AND ANTIBACTERIAL METABOLITE FROM MICROBIAL ISOLATE; STREPTOMYCES RIMOSUS MTCC 10792 FROM SOIL OF CHHATTISGARH

摘要

A microbial isolate, isolated from forest soil of Chhattisgarh, (India) was found to show significant antimicrobial activity against gram positive and gram negative bacteria as well as filamentous fungi including human pathogens. Based on physiological, biochemical characteristics the isolate was identified as Streptomyces rimosus subsp. rimosus and score id was calculated by the PIBWin software 1.9.2. This strain was further cultivated in different conditions fermentation in order to optimize biosynthetic process of antibiotic production including: different pH values, different temperatures, different carbon and nitrogen sources were fully investigates. Minimum inhibitory concentration of the crude extract was found ranged between 6.25-12.5μg/ml, 12.5- 25μg/ml and 25-50μg/ml against the filamentous, unicellular fungal and bacterial test strains. This strain is a found to be a potential antibiotic producer strain and may be commercially exploited for the development of novel drugs