A new Bacterial strain Streptomyces indiaensis (LMG 19961) and its larvicidal and Histopathological effect against Anopheles stephensi: A Malaria Mosquito

摘要

The present study was carried out to establish larvicidal activity (LC50 determination) and mode of action of a soil bacteria Streptomyces indiaensis strain (LMG 19961)by taking midgut as a target tissue against third instar larvae of Anopheles stephensi (L.), the vector of malaria. LC_(50) of Streptomyces indiaensis was calculated by biotoxicity test. The LC_(50) (1.645 cfu/ml) treated third instar larvae showed dose dependent effect on mortality. The histoarchitecture of midgut revealed weak and disoriented epithelial cells with larger intercellular spaces and disruption of junctional complexes. The lysed gastric caecal cells were also noticed and reduced lumen further revealed the severity of bacterial strain against mosquito larvae. The peritrophic membrane was dislodged from the place. Since this strain of Streptomyces indiaensis was reported first time in our laboratory (NCBI Accession number KJ170314) the probability of ecosafe vector control agents from soil bacteria is quite encouraging.