IN VITRO FREE RADICAL SCAVENGING AND ANTICANCER POTENTIAL OF ARISTOLOCHIA INDICA L. AGAINST MCF-7 CELL LINE

摘要

Objective: To investigate the in vitro free radical scavenging and anticancer potential of Aristolochia indica L . leaves and stem against MCF-7 cell line. Methods: Phytochemicals were analysed in chloroform (leaves) and aqueous extracts (stem) of the Aristolochia indica by using standard methods. In vitro antioxidant studies were carried out for the chloroform and aqueous extracts of the Aristolochia indica using various free radical models such a DPPH, Reducing power assay, hydrogen peroxide (H 2 O 2 ) scavenging,. In vitro cytotoxic assay such as MTT assays were carried out against MCF-7 cell line. Results: Preliminary phytochemical screening revealed the presence of flavonoids, tannins, glycosides, phenol, saponins, in chloroform extract of leaves and aqueous extract of stems. The results revealed that the chloroform extract has significant antioxidant potential than aqueous extract. The result revealed that the chloroform extracts of Aristolochia indica L showed pronounced anticancer activity against Ehrlich Ascites Carcinoma (MCF-7) cell line than ethanol extract. Conclusion: The result of the present study concluded that the chloroform extract of Aristolochia indica L has significant antioxidant and anticancer activity then the aqueous extract. The potential antioxidant and anticancer activity of Aristolochia indica L might be due to the presence of phytochemicals. Keywords: Aristolochia indica L, Ehrlich Ascites Carcinoma, Free radicals, Antioxidant.